Utility of cysticercus fasciolaris antigen in Dot ELISA for the diagnosis of neurocysticercosis
نویسندگان
چکیده
منابع مشابه
Utility of cysticercus fasciolaris antigen in Dot ELISA for the diagnosis of neurocysticercosis.
BACKGROUND Clinical diagnosis of neurocysticercosis (NC) is established by CT scan and MRI. However, absolute diagnosis is not possible in a fair number of cases, and serological assays are used as adjunct. Besides, CT scan and MR imaging are resource-intensive tests and not practical for screening in endemic areas. AIM To provide a low-cost, efficient, and reproducible assay for the detectio...
متن کاملDot-ELISA for the diagnosis of neurocysticercosis.
The aim of the present study was to standardize and evaluate dot-Enzyme linked immunosorbent assay (Dot-ELISA), a simple and rapid test for the detection of cysticercus antibodies in the serum for the diagnosis of neurocysticercosis (NCC). The antigen used in the study was a complete homogenate of Cysticercus cellulosae cysts obtained from infected pigs and dotted on to nitrocellulose membrane....
متن کاملThe Chemistry of Cysticercus Fasciolaris
The sarcomas arising in rat livers at the surface of the cysts surrounding the tapeworm larvae, Cysticercus fasciolaris (Taeniu taeniaformis), have been extensively studied by Curtis, Dunning, and Bullock, (1). Recently in this laboratory, as part of a cooperative investigation sponsored by The International Cancer Research Foundation, a chemical investigation of the extractable components of t...
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Comparison of the Utility of Recombinant B8/2 Subunit of the Antigen B, Native Antigen, and a Commercial ELISA Kit in the Diagnosis of Human Cystic Echinococcosis
Background: Cystic echinococcosis (CE) is a helminthic disease caused by the larval form of Echinococcus granulosus. In the present study, the B8/2 subunit of antigen B (AgB) of E. granulosus was expressed in E. coli host and then applied in a diagnostic ELISA set up. Methods: The DNA sequence of AgB8/2 subunit from E. granulosus was extracted from the GenBank and codon-optimized. The target s...
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ژورنال
عنوان ژورنال: Indian Journal of Medical Sciences
سال: 2008
ISSN: 0019-5359
DOI: 10.4103/0019-5359.41608